Journal: Cancers

Article Title: A Deep Learning Model for Cervical Cancer Screening on Liquid-Based Cytology Specimens in Whole Slide Images

doi: 10.3390/cancers14051159

Figure Lengend Snippet: Representative manually drawing annotation images for neoplastic labels on liquid-based cytology (LBC) slides. The LBC case ( A ) was diagnosed as HSIL (high-grade squamous intraepithelial lesion) based on the representative neoplastic squamous epithelial cells with increase in nuclear/cytoplasmic ratio and nuclear atypia ( B – D ). The LBC case ( E ) was diagnosed as SCC (squamous cell carcinoma) based on the representative neoplastic squamous epithelial cells with HSIL features ( F – H ). Representative neoplastic cells were roughly annotated using in-house on-line drawing tools.

Article Snippet: A total of 3121 LBC ThinPrep Pap test (Hologic, Inc.) conventionally prepared cytopathological slide glass specimens of human cervical cytology were collected from a private clinical laboratory in Japan after cytopathological review of those specimens by cytoscreeners and pathologists.

Techniques:

Journal: Cancers

Article Title: A Deep Learning Model for Cervical Cancer Screening on Liquid-Based Cytology Specimens in Whole Slide Images

doi: 10.3390/cancers14051159

Figure Lengend Snippet: A representative example of neoplastic true positive prediction outputs on a liquid-based cytology (LBC) case from test sets. In the neoplastic whole-slide image (WSI) of LBC specimen ( A ), the heatmap image ( B ) shows a true positive prediction of neoplastic epithelial cells in high probability tiles ( C , D ), which correspond, respectively, to neoplastic epithelial cells ( E – G ) equivalent to HSIL (high-grade squamous intraepithelial lesion). On the other hand, in low probability tiles ( H , I ) of the same heatmap image ( B ), there are no evidence of neoplastic cells.

Article Snippet: A total of 3121 LBC ThinPrep Pap test (Hologic, Inc.) conventionally prepared cytopathological slide glass specimens of human cervical cytology were collected from a private clinical laboratory in Japan after cytopathological review of those specimens by cytoscreeners and pathologists.

Techniques:

Journal: Cancers

Article Title: A Deep Learning Model for Cervical Cancer Screening on Liquid-Based Cytology Specimens in Whole Slide Images

doi: 10.3390/cancers14051159

Figure Lengend Snippet: A representative example of neoplastic true negative prediction outputs on a liquid-based cytology (LBC) case from test sets. In the NILM (negative for intraepithelial lesion or malignancy) whole slide image (WSI) of LBC specimen ( A ), the heatmap image ( B ) shows true negative prediction of neoplastic epithelial cells which correspond, respectively, to non-neoplastic epithelial cells ( C ). Moreover, in very low probability tiles ( D , E ) of the same heatmap image ( B ), there are no evidence of neoplastic cells.

Article Snippet: A total of 3121 LBC ThinPrep Pap test (Hologic, Inc.) conventionally prepared cytopathological slide glass specimens of human cervical cytology were collected from a private clinical laboratory in Japan after cytopathological review of those specimens by cytoscreeners and pathologists.

Techniques:

Journal: Cancers

Article Title: A Deep Learning Model for Cervical Cancer Screening on Liquid-Based Cytology Specimens in Whole Slide Images

doi: 10.3390/cancers14051159

Figure Lengend Snippet: A representative example of neoplastic false positive prediction outputs on a liquid-based cytology (LBC) case from test sets. Cytopathologically, ( A ) is a NILM (negative for intraepithelial lesion or malignancy) whole-slide image (WSI) of LBC specimen. The heatmap image ( B ) exhibited false positive predictions of neoplastic tiles ( C , E ). In ( C ), there are parabasal cells with a slightly high nuclear cytoplasmic (N/C) ratio with dense chromatin appearance due to the cellular overlapping ( D ). In ( E ), there are cell clusters of squamous epithelial cells and cervical gland cells with slightly high N/C ratios and a dense chromatin appearance due to the cellular overlapping.

Article Snippet: A total of 3121 LBC ThinPrep Pap test (Hologic, Inc.) conventionally prepared cytopathological slide glass specimens of human cervical cytology were collected from a private clinical laboratory in Japan after cytopathological review of those specimens by cytoscreeners and pathologists.

Techniques:

Journal: Oncotarget

Article Title: Cytomorphological characteristics of glassy cell carcinoma of the uterine cervix: histopathological correlation and human papillomavirus genotyping

doi: 10.18632/oncotarget.12361

Figure Lengend Snippet: A. Thinprep preparation of the cervical sample shows amphophilic, granular necrotic debris (tumor diathesis) and a few small clusters of polygonal tumor cells. B. The tumor cell clusters or individually scattered tumor cells are unevenly distributed. Tumor diathesis is apparent. C. Although some tumor cells display endocervical-like pseudocolumnar arrangements (black arrowheads), there is no definite evidence of glandular differentiation. A white arrow indicates the intercellular window. D. The tumor cells have relatively fine chromatin and prominent, solitary nucleoli. Abundant, cyanophilic cytoplasm and discrete cell borders (white arrows) are evident. E. Under high-power magnification (×400), the tumor cells show large, oval to round, pleomorphic nuclei and “intercellular windows” produced by discrete cytoplasmic outlines and cytoplasmic molding (white arrow). There are no intercellular bridges. F. Tumor cells are 3–7 fold larger than lymphocytes or neutrophils. Chromatin distribution irregularities, hyperchromasia, and significant anisonucleosis are apparent. G-I. In several areas, an intimate admixture of neutrophils (red circles) and tumor cells, so-called granuloepithelial complexes, is seen. Cytoplasmic molding and intercellular windows (white arrows) are observed. J-K. Mitotic figures (blue arrows) are present. L. Atypical mitotic figures (red arrow) are also detected (A-L, Papanicolaou stain).

Article Snippet: The ThinPrep test (Hologic Inc., Marlborough, MA, USA) was performed using an automated liquid-based monolayer cell preparation system (ThinPrep 2000 system; Hologic Inc.), according to manufacturer's recommendations.

Techniques: Produced, Papanicolaou Stain